Nowadays, stem cells, beside their research aspects, have been considered a practical tool to improve treatment methods. However, despite all of breakthroughs in this research, reliability of this approach is still a question mark when compared with other clinical methods. Lack of proper migration and implantation (homing) of stem cells toward the damaged tissues (target) is one of the most obvious problems for suitable cell therapy. Various factors are involved in this ineffectiveness of homing such as defects on CXCR4-SDF-1axis etc. It has been realized that SDF-1 is one of the most important chemokine involved in recruitment and increased migration of stem cells to the desired tissues. Therefore, procedures stimulating SDF-1 gene expression in damaged tissues are required. So, in the current study, it has been hypothesized that both hypoxia and low level laser therapy (LLLT) may increase the expression and secretion of chemokine SDF-1 which may help to enhance stem cells recruitment. To test this hypothesis, muscle cells were treated to be hypoxic via physical effect such as laser treatment. The optimum treatment conditions were observed by investigating the cellular viability (MTT Assay) after treatment. Real-Time PCR and Western blotting analysis were done to study the SDF-1 expression in treated cells. Migration assay was performed to test the migration potential of MSCs toward Hypoxic cells. It was observed that 4 hours Hypoxic incubation time and 12 joules per square centimeter of laser treatment are the optimum conditions for muscle cells. Real-Time PCR showed the 10 fold SDF-1 expressionin cells after 4 hours of incubation in hypoxic condition and 72 hours of normoxic conditions. Western blotting results confirmed the expression of SDF-1 at protein level. Migration assay also showed the migration of MSCs towards hypoxic cells. It can be concluded that hypoxic and laser treatment of cells can recruit MSCs and can be applied a strategy for the targeted stem cell transplantation.