Bactrian camel was domesticated 2500 BCE, probably in northern Iran. Iranian Bactrian camel (Two Hump camel) lives in northwest of Iran (Ardebil province) and they are one of the endangered livestock breeds. Preserved and registered this breed for Iran is the first researcher missions. In this project, sampling was done from people flock and research institutions in Ardebil province. Ear skin tissue was sampled from 65 Bactrian camel and cultured in high glucose DMEM. We cultured Bactrian camel fibroblasts in vitro, analyzed their biological characteristics, chromosomes and cell cycles. Twenty four hours, 4, 7 and 14 days after seeding, the number of cells assessed and growth curve was plotted. 7 cell lines were removed due to non- proliferation and infection observed and 58 cell lines were generated. Tests for cell lines contamination with bacteria, fungi, or mycoplasmas were also negative. For all samples, Chromosome images (karyotyping), birth certificate, cells image, animal photo, breed and horses Specifications were taken. The results indicated that Bactrian camel ear fibroblasts can be successfully obtained by tissue culture and cell line creation of end angered species is one of the most functional, fastest and economic always to preserved genetic resources.